Seminar RNAscope technique

Publié le 24/07/2023

Tuesday 19 September at 13:30, Laurent Vinay meeting room.

Seminar to introduce you to the RNAscope technique.

* This technique can be used to localise different types of RNA (mRNA, microRNA, etc.) on cells or tissue. This can be an alternative when antibodies are not available, but also enables dual RNA/protein analysis, since RNAscope is compatible with the immunolabelling technique.

* If you have an antibody that works in immunohistochemistry, combining it with RNAscope opens up the possibility of visualising gene expression at both mRNA and protein levels on the same sample (the presence and quantity of mRNA not necessarily being a reflection of protein expression).

* Multiplexing can also be used to characterise different cell populations, for example.

Glial- and Neuronal-Specific Expression of CCL5 mRNA in the Rat Brain  https://www-ncbi-nlm-nih-gov.insb.bib.cnrs.fr/pmc/articles/PMC5770405/pdf/fnana-11-00137.pdf

A method for combining RNAscope in situ hybridization with immunohistochemistry in thick free-floating brain sections and primary neuronal cultures  https://journals-plos-org.insb.bib.cnrs.fr/plosone/article/file?id=10.1371/journal.pone.0120120&type=printable

Molecular and anatomical characterization of parabrachial neurons and their axonal projections  https://elifesciences.org/articles/81868.pdf